Composite

Part:BBa_K3904005:Design

Designed by: Ita Čiutaitė   Group: iGEM21_Vilnius-Lithuania   (2021-09-28)


Bile-regulated and cold-inducible VapXD kill-switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 199
    Illegal BamHI site found at 397
    Illegal XhoI site found at 442
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 179
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part's performance depends on temperature and bile salts.